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Whole genome resequencing
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Obtain higher genome coverage and identify variations. |
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Long (250-base) reads effectively span many sequences that micro-reads interpret as repeats and eliminate the need to filter against a reference. |
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Bibliography |
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De novo whole genome sequencing and BAC sequencing
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Obtain high-quality, comprehensive assemblies and minimize costly follow-up experiments such as gap closure. |
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Scaffolded assemblies with >99% accuracy are possible, in addition to approximately 10-fold fewer contigs and minimal mis-assemblies. |
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Bibliography |
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Transcriptome assembly
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Sequence and assemble the transcriptome of very complex organism (e.g., plants) as an alternative to de novo genome sequencing. |
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Long reads provide enough contiguous sequence to de novo assemble unknown transcriptomes, identify splice variants, and annotate transcripts of unknown function. |
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Bibliography |
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Amplicon sequencing
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Identify low-frequency mutations and haplotypes over the full exon length. |
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Reads from single DNA molecules span typical amplicons such as exons. |
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Bibliography |
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Non-coding RNA studies
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Generate a more complete inventory and perform functional analysis of non-coding RNAs in the genome. |
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Long reads cover the range of non-coding RNA (ncRNA) species (from 20 nt to 300 nt) and 99.5% single-read accuracy enables differentiation of highly similar sequences. |
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Bibliography |
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