Services

Pushing the boundaries of technology and knowledge

We are pioneers in massive genome sequencing technology, focusing on microbial biotechnology and implications of microbiome and health. Having in-house sequencing capabilities allow us to rapidly iterate our developments when looking for novel biological activities

Discover our services.

NGS Projects for the microbial world

NGS Projects for humans

NGS Projects for food genomics

NGS Projects for animals

Using NGS it is possible to investigate the composition of microbial communities by sequencing regions of taxonomic value such as the 16S subunit gene of rRNA for the study of bacteriome. This gene is composed of highly conserved regions interspersed with more variable regions. In ADM Lifesequencing we have a great experience in metagenomics of the 16S rRNA. During all these years we have sequenced more than 45,000 samples in different projects, with clients from all over the world and analyzing very diverse samples that have allowed us to optimize and give added value to our analysis of microbial diversity. Knowing the appropriate coverage, the optimum reading quality and the maximum length to be obtained, allows us to detect bacteria that are in lower proportion and the correct taxonomic assignment. This has led us to develop our own optimized protocol so that you can achieve the best results in the most cost-efficient way. Our bioinformatics team also has a great deal of experience and has developed its own database.

Although the study of microbial diversity through the capture by PCR of the regions of the rRNA 16S gene are the most well-known and performed, there are also other types of genes specific to other groups of organisms:

  • Analysis of fungal diversity (filamentous fungi and yeasts) by capturing the intergovernmental region between the genes of rRNA subunits 5.4S and 18S.

ADM Lifesequencing has a great diversity of optimized protocols for different communities of organisms that will allow us to isolate DNA from any type of sample you provide us such as water, soil, digestive tract, food, clinical samples, etc.

Thanks to this technique you will be able to:

  • Analyze the variation of species in a complex sample being able to ascribe taxonomically to the organisms that conform it (fungi and bacteria).
  • Establish interspecific comparisons.
  • Studies of population dynamics, monitoring of processes.
  • Characterize biomarkers for specific environments

Metagenomics, also called environmental genomics or community genomics, refers to the study of communities living in different habitats, without the need to isolate them beforehand and grow cultures in controlled environments. Thus, we can obtain information at both the community level and also on its potential gene functions. With this approach there is no difference between organisms that have an active participation in metabolic fluxes and those that are present in the community but inactive.

With NGS technology we can obtain a high number of sequences in parallel and a great coverage that allows us to have a significant representation of the bacterial diversity. We can also sequence different samples in the same sequencing run, for most cost-effective experiments.

This approach will allow you to:

  • Collect the potential and genetic richness of a community of organisms at a functional level, predicting metabolic networks.
  • To establish interspecific comparisons and evaluation of genes/enzymatic activities of biotechnological interest.
  • Characterization of biomarkers for specific environments.

ADM Lifesequencing offers the latest NGS techniques to analyze RNAs of any type of sample and analyzing thousands of genes in order to obtain a global image of the cell function both in microorganisms and eukaryotic organisms. We can study changes that occur in pathological situations, in response to certain drugs or even under different environmental conditions. Transcriptomics can be also applied to the RNA study of a community of organisms in order to analyze the expression of those genes involved in the interaction between them or, through the study of ribosomal RNA, to taxonomically identify the living organisms of the concrete ecosystem.

Thanks to this technique you will be able to:

  • Recognize functional genes of interest (e.g. vitamin production, antibiotics).
  • Identify new metabolic pathways involved genes.
  • Detect isoforms or variants of mRNA.
  • Quantify RNA molecules, thus obtaining a profile of gene expression.
  • Identify gene fusion, variants of a single nucleotides
  • Identify viable microorganisms

ADM Lifesequencing has great experience in the sequencing of small, medium and large genomes. Depending on the type of genome, complexity and size we can advise you and offer you the most economically efficient sequencing strategy. We will provide you with comprehensive advice, from start to finish of your project.

Thanks to this technique you will be able to:

  • Facilitate and better understand your molecular studies
  • Generation of high-quality reference sequences for complex genomes.
  • Useful information for the mapping of new organisms or the closure of known genomes.
  • Identification of structural variants and regroupings such as deletions, inversions or translocations.

ADM Lifesequencing has carried out projects for sequencing microbial de novo genomes using different NGS platforms.

Metagenomics in humans (biopsies, scrapes, sputum and/or exudates), refers to microorganism genes located in areas affected by some pathology. It is useful when the causal agent of the symptoms is unknown. Using MGS it is possible to research on microbial communities at a functional and taxonomic level

If a specific microbial group is suspected, it is possible to use the LIFE-BIOME service to identify bacteria or fungi and yeasts and determine the presence of possible pathogenic microorganisms. It is also possible the metatrascriptomic approach to identify the active mechanisms of pathogenicity and defense of the host.

Sequencing an entire genome can be complicated when there is a lot information to be obtained. To avoid these problems, the solution adopted consists on sequencing the gene related to the symptomatology of the patient. This is a great tool because of many reasons:

  • Genetic advice is much simpler, given that all the findings are related to the pathology of interest.
  • The probability of alterations of unknown significance is much lower.
  • By sequencing fewer nucleotides, greater coverage can be achieved, resulting in fewer false positives.
  • We obtain less data, which means a lower need for bioinformatics infrastructure.
  • We avoid casual findings.

Therefore, the sequencing of gene panels is usually the preferred approach for the study of diseases of multigenic origin. We also have the possibility of producing custom sequencing panels.

Exome sequencing is based on the selective sequencing of regions of the genome. It is the functional part that contributes to the final phenotype of an organism. The characterization of the complete exome of an individual allows the search and identification of genes causing disease, as well as the determination of variants that increase the risk of suffering certain diseases. Currently there are different capture kits including more or less coverage of human exons and even including other targets of interest such as small RNAs. Consult our specialists for more information

The human genome is the complete set of chromosomes that make up an individual with its associated genes, that is, all the genetic material (DNA) of an individual. Currently there are mass sequencing kits that allow the entire human genome to be sequenced in a few days at a much reduced price. This places personalized genetic analysis at the threshold of what is considered an affordable cost in clinical use. Sequencing is particularly useful in the diagnosis of rare hereditary diseases or those diseases with unknown mutations. Consult our specialists for more information.

Since the characterization of the human genome, new avenues of research on the global analysis of genetic material have emerged. The concept of transcriptome arises to represent mRNA transcribed in certain circumstances. ADM Lifesequencing can provide a comparison of the differential expression of a series of individuals phenotypically different or exposed to different conditions that will provide the doctor with information on the transcriptomics of the patient. Consult our specialists for more information.

Small RNA has less than 200 nucleotides in length and is usually non-coding RNA molecules. The most common function of these molecules is gene regulation, by silencing RNA with complementary sequences, inducing degradation of messenger RNA. Lately, the study of microRNAs, which are smallRNAs of only 21-26 nucleotides in size, has gained great importance through the massive sequencing of these molecules.

At ADM Lifesequencing we offer you the possibility to scrutinize small RNAs by means of massive genomic sequencing. This method has become one of the most widely used for analyzing human cells and has recently taken on great importance by locating targets to design therapeutic treatments for rare and hereditary diseases. Consult our specialists for more information.

It is possible to design strategies to evaluate the methylation changes of specific areas of the genome already known or to be discovered. Using a PCR capture approach, it is possible to reduce costs and evaluate by massive genomic sequencing the promoter zones of the genes under study. Consult our specialists for more information.

With the new ddPCR platform (digital PCR) there are many applications we can offer you in the clinical area. ADM Lifesequencing has the following products validated for detection of mutations or determination of the number of copies:

ddPCR Multiple/ Gen Detected mutations
BRAF V600 V600E, V600K, V600R
KRAS G12/G13 G12A, G12C, G12D, G12R, G12S, G12V, G13D
KRAS Q61 Q61H (183A>C), Q61H (183A>T), Q61K, Q61L, Q61R
NRAS G12 G12A, G12C, G12D, G12R, G12S, G12V
NRAS G12/G13 G12A, G12C, G12D, G12S, G12V, G13D, G13R, G13V
NRAS Q61 Q61H (183A>C), Q61H (183A>T), Q61K, Q61L, Q61R
EGFR exon19 deletions >15 EGFR exon19 deletions

 

Gene Lung Cancer Colorectal Cancer Ovariy Cancer Tiroides Cancer Melanoma G Glioma Pancreas Cancer
BRAF
KRAS
NRAS
EGFR

 

In addition, as a result of the developments of international research groups, we have a database to find the perfect target for your research studies:

  • Detection of rare mutations.
  • Detection of mutations in silica.
  • Differential expression
  • Multiple copy gene variations.

Contact us for more information.

 

We can provide you in a very short period of time the sequencing for clinical applications for cases of infections or pathologies that need a quick response.

ADM Lifesequencing offers its customers a food fraud detection service for ingredients and food through massive genomic sequencing and bioinformatics analysis.

The quality and economic value of a food product is directly proportional to its composition, for example through the use of species or varieties with improved organoleptic qualities or mixtures of components that after a greater industrial processing can provide better characteristics.

Nowadays, using molecular biology techniques, it is possible to identify the species that compose a food and their proportion, being able to solve highly processed foods. ADM Lifesequencing has developed a set of specific services for the agro-food industry, with which it is possible to identify the variants or species that are part of processed foods and certify the composition of products in detail:

  • FoodDNAInspector microbial diversity, to monitor fermented foods or directly analyze the microbial component of the product.
  • FoodDNAInspector plant extracts, used in comparison of highly processed products where it is difficult to know the different aromatic plants used.
  • FoodDNAInspector meat and dairy products, for meat or dairy mixtures where it is important to ensure the absence of products such as pork or horse or high value species such as duck.
  • FoodDNAInspector seafood products, used to identify the species used and verify the composition of the label.

ADM Lifesequencing extracts the DNA of the product and by joining the techniques of massive genomic sequencing and bioinformatics analysis, mainly using constitutive genes specific to each group, for example, the gene of the subunit 18S of rRNA, the gene rblc located in chloroplasts or mitochondrial genes, an analytical report is prepared with the composition of genera and species that compose the food or product to be evaluated. It is also possible to compare the different stages of processing of a food product.

When evaluating why an animal or a plant variety has superior characteristics to its counterparts and you want to identify them, it is useful to know the differences in the genes that makes it particular compared to other individuals.

The concept of genotyping by mass sequencing arises in order to be able to analyze a large number of individuals at an appropriate cost and identify individuals or groups of individuals with special characteristics as a genetic fingerprint without having the whole genome sequenced. Consult our specialists for more information.

When evaluating why an animal or plant product has superior characteristics to its counterparts, it is useful to know the differences between them.  Thus, a challenge is to study what portion of the genome is transcribed into messenger RNA differential between foods, that is, what genetic material is expressed into proteins under a cell type under given conditions.

The concept of transcriptome arises to represent all this mRNA transcribed in certain circumstances, in a global way. There are countless transcripts depending on the tissue type or environmental conditions even for the same species. ADM Lifesequencing can provide a comparison of the differential expression of a series of individuals phenotypically different or exposed to different conditions that will provide the physician with information on the transcriptomics of the patient. Consult our specialists for more information.

In addition, we can provide you in a very short period of time the sequencing for agri-food applications in cases of food fraud that need a quick response.

With the new ddPCR platform there are many applications that we can offer you in the agrifood area, from the detection of pathogens and food alterants to the quantification of specific compounds or ingredients. For more information, please contact our specialized staff.

Since the characterization of the human genome, new avenues of research on the global analysis of genetic material have emerged. The concept of transcriptome arises to represent mRNA transcribed in certain circumstances. ADM Lifesequencing can provide a comparison of the differential expression of a series of individuals phenotypically different or exposed to different conditions that will provide the doctor with information on the transcriptomics of the patient. Consult our specialists for more information

Small RNA has less than 200 nucleotides in length and is usually non-coding RNA molecules. The most common function of these molecules is gene regulation, by silencing RNA with complementary sequences, inducing degradation of messenger RNA. Lately, the study of microRNAs, which are smallRNAs of only 21-26 nucleotides in size, has gained great importance through the massive sequencing of these molecules.

At ADM Lifesequencing we offer you the possibility to scrutinize small RNAs by means of massive genomic sequencing. This method has become one of the most widely used for analyzing human cells and has recently taken on great importance by locating targets to design therapeutic treatments for rare and hereditary diseases. Consult our specialists for more information.

It is possible to design strategies to evaluate the methylation changes of specific areas of the genome already known or to be discovered. Using a PCR capture approach, it is possible to reduce costs and evaluate by massive genomic sequencing the promoter zones of the genes under study. Consult our specialists for more information.

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